Description
Boster’s human CXCL7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for CXCL7 has been precoated onto 96-well plates. Standards(E.coli, A59-D128) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL7 amount of sample captured in plate.
Synonyms: B TG1|_x000D_Beta TG|_x000D_Beta thromboglobulin|_x000D__x000D__x000D_C X C motif chemokine 7|_x000D_Connective tissue activating peptide III|_x000D_CTAP III|_x000D_CTAP3|_x000D_CTAPIII|_x000D_LA PF4|_x000D_LDGF|_x000D_Leukocyte derived growth factor|_x000D_Low affinity platelet factor IV|_x000D_Macrophage derived growth factor|_x000D_MDGF|_x000D_NAP 2|_x000D_Neutrophil activating peptide 2|_x000D_PBP|_x000D_Platelet basic protein|_x000D_Ppbp|_x000D_pro platelet basic protein (chemokine (C X C motif) ligand 7)|_x000D_SCYB7|_x000D_Small inducible cytokine B7|_x000D_Small inducible cytokine subfamily B, member 7|_x000D_TC1|_x000D_TC2|_x000D_TCK 1|_x000D_TGB|_x000D_TGB1|_x000D_THBGB|_x000D_THBGB1|_x000D_Thrombocidin 1|_x000D_Thrombocidin 2|_x000D_Thymus Chemokine 1
Species: Human CXCL7
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <2pg/ml Specifity: Natural and recombinant human CXCL7
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, A59-D128
Gene name: C-X-C motif chemokine 7 / pro-platelet basic protein
Protein name: C-X-C motif chemokine 7
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|29|155|411|28|157|395
Standard deviation|1.47|7.9|29.18|1.73|9.26|32.78
CV(%)|5.1%|5.1%|7.1%|6.2%|5.9%|8.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 29 | 31 | 30 | 30 | 30 | 0.7 | 2.3% |
| Sample 2 | 155 | 156 | 165 | 162 | 159 | 4.15 | 2.6% |
| Sample 3 | 411 | 409 | 455 | 402 | 419 | 20.9 | 4.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0729-CAP|96-well plate precoated with anti- human CXCL7 antibody|1
EK0729-ST|Lyophilized recombinant human CXCL7 standard|10ng/tube×2
EK0729-DA|Biotinylated anti- human CXCL7 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
