Description
Boster’s human CXCL13 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL13 has been precoated onto 96-well plates. Standards(E.coli, V23-R94) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL13 amount of sample captured in plate.
Synonyms: ANGIE|ANGIE2|B cell attracting chemokine 1|B cell chemoattractant|B cell homing chemokine (ligand for Burkitt’s lymphoma receptor 1)|B lymphocyte chemoattractant|BCA 1|BLC|BLR1L|C X C motif chemokine 13|Chemokine (C X C motif) ligand 13|Chemokine (C X C motif) ligand 13 (B cell chemoattractant)|Chemokine CXC motif ligand 13|CXC chemokine BLC|CXCL13|SCYB13|Small inducible cytokine B subfamily (Cys X Cys motif) member 13 (B cell chemoattractant)|Small inducible cytokine B13|Small inducible cytokine subfamily B, member 13
Species: Human CXCL13
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant human CXCL13
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, V23-R94
Gene name: C-X-C motif chemokine 13 / chemokine (C-X-C motif) ligand 13
Protein name: C-X-C motif chemokine 13
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|32|216|441|30|232|416
Standard deviation|2.2|16.84|24.69|2.19|20.64|28.28
CV(%)|6.9%|7.8%|5.6%|7.3%|8.9%|6.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 32 | 31 | 36 | 31 | 32 | 2.06 | 6.4% |
| Sample 2 | 216 | 244 | 229 | 223 | 228 | 10.31 | 4.5% |
| Sample 3 | 441 | 424 | 416 | 416 | 424 | 10.2 | 2.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0739-CAP|96-well plate precoated with anti- human CXCL13 antibody|1
EK0739-ST|Lyophilized recombinant human CXCL13 standard|10ng/tube×2
EK0739-DA|Biotinylated anti- human CXCL13 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
