Description
Boster’s human CXCL10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL10 has been precoated onto 96-well plates. Standards(E.coli, V22-P98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL10 amount of sample captured in plate.
Synonyms: 10 kDa interferon gamma-induced protein|C7|Chemokine (C X C motif) ligand 10|Chemokine CXC motif ligand 10|Crg 2|CRG2|CXCL10|CXCL10(1-73)|CXL10_HUMAN|Gamma IP10|Gamma-IP10|gIP 10|GIP10|IFI10|INP 10|Interferon activated gene 10|Interferon gamma induced cell line|Interferon inducible cytokine IP10|IP 10|IP-10|MOB1|SCYB10|Small inducible cytokine B10|Small inducible cytokine B10 precursor|Small inducible cytokine subfamily B (Cys X Cys) member 10|Small inducible cytokine subfamily B CXC member 10|Small-inducible cytokine B10
Species: Human CXCL10
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant human CXCL10
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, V22-P98
Gene name: C-X-C motif chemokine 10 / chemokine (C-X-C motif) ligand 10
Protein name: C-X-C motif chemokine 10
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|60|243|879|64|223|865
Standard deviation|3.24|19.19|45.7|4.28|19.4|47.57
CV(%)|5.4%|7.9%|5.2%|6.7%|8.7%|5.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 60 | 54 | 60 | 64 | 59 | 3.57 | 6% |
| Sample 2 | 243 | 258 | 258 | 260 | 254 | 6.83 | 2.6% |
| Sample 3 | 879 | 923 | 848 | 978 | 907 | 48.89 | 5.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0735-CAP|96-well plate precoated with anti- human CXCL10 antibody|1
EK0735-ST|Lyophilized recombinant human CXCL10 standard|10ng/tube×2
EK0735-DA|Biotinylated anti- human CXCL10 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
