Description
Boster’s human complement C5a ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for complement C5a has been precoated onto 96-well plates. Standards(E.coli, T678-R751) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for complement C5a is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human complement C5a amount of sample captured in plate.
Synonyms: anaphylatoxin C5a analog|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4|C5|CO5_HUMAN|complement C5|Complement C5 alpha chain|Complement component 5|CPAMD4|ECLZB
Species: Human complement C5a
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human complement C5a
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, T678-R751
Gene name: Complement C5 / complement component 5
Protein name: Complement C5
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|89|285|1037|84|285|1015
Standard deviation|4.09|17.1|66.36|5.12|21.09|69.02
CV(%)|4.6%|6%|6.4%|6.1%|7.4%|6.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 89 | 97 | 98 | 84 | 92 | 5.78 | 6.2% |
| Sample 2 | 285 | 261 | 256 | 258 | 265 | 11.68 | 4.4% |
| Sample 3 | 1037 | 990 | 926 | 1010 | 990 | 40.93 | 4.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0966-CAP|96-well plate precoated with anti- human complement C5a antibody|1
EK0966-ST|Lyophilized recombinant human complement C5a standard|10ng/tube×2
EK0966-DA|Biotinylated anti- human complement C5a antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
