Description
Boster’s human CD10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD10 has been precoated onto 96-well plates. Standards(CHO, Y52-W750) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD10 amount of sample captured in plate.
Synonyms: Atriopeptidase|CALLA|CD 10|CD10|CD10 antigen|Common acute lymphocytic leukemia antigen|DKFZp686O16152|EC 3.4.24.11|Enkephalinase|EPN|gp100|Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase)|Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10)|Membrane metallo endopeptidase|Membrane metallo endopeptidase variant 1|Membrane metallo endopeptidase variant 2|Membrane metalloendopeptidase|Membrane metalloendopeptidase neutral endopeptidase enkephalinase|Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10|Membrane metalloendopeptidase variant 1|Membrane metalloendopeptidase variant 2|MGC126681|MGC126707|MME|NEP|NEP_HUMAN|Neprilysin|Neutral endopeptidase 24.11|Neutral endopeptidase|SFE|Skin fibroblast elastase
Species: Human CD10
Assay: 125pg/ml-8000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CD10
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: CHO, Y52-W750
Gene name: Neprilysin / membrane metallo-endopeptidase
Protein name: Neprilysin
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|201|956|4316|216|881|4302
Standard deviation|14.87|51.62|233.06|17.92|60.78|305.44
CV(%)|7.4%|5.4%|5.4%|8.3%|6.9%|7.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 201 | 170 | 181 | 181 | 183 | 11.18 | 6.1% |
| Sample 2 | 956 | 1058 | 1011 | 1064 | 1022 | 43.4 | 4.2% |
| Sample 3 | 4316 | 4490 | 4532 | 4435 | 4443 | 81.12 | 1.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0851-CAP|96-well plate precoated with anti- human CD10 antibody|1
EK0851-ST|Lyophilized recombinant human CD10 standard|10ng/tube×2
EK0851-DA|Biotinylated anti- human CD10 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
