Human CCL22/MDC PicoKine™ Fast ELISA Kit

Human CCL22/MDC PicoKine™ Fast ELISA Kit

Brand:
Boster Bio
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The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human CCL22 with <1pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. CCL22 tissue specificity: Highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis. .   

 

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SKU: FEK0446 Category:

Description

Boster’s human MDC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MDC has been precoated onto 96-well plates. Standards(E.coli, G25-Q93) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MDC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MDC amount of sample captured in plate.

Synonyms: C-C motif chemokine 22;CC chemokine STCP-1;MDC(1-69);Macrophage-derived chemokine;Small-inducible cytokine A22;Stimulated T-cell chemotactic protein 1;MDC(3-69);MDC(5-69);MDC(7-69);CCL22;MDC, SCYA22;A-152E5.1;

Species: Human MDC

Assay: 31.2pg/ml-2000pg/ml

Sensitivy: <1pg/ml Specifity: Natural and recombinant human MDC

Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat

Anticoagulant: heparin or EDTA

Immunogen: Expression system for standard: E.coli; Immunogen sequence: G25-Q93

Gene name: C-C motif chemokine 22 / C-C motif chemokine 22

Protein name: C-C motif chemokine 22

Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 16 16 16 24 24 24
Mean(pg/ml) 97 212 842 87 210 866
Standard deviation 7.37 9.11 55.57 7.91 12.6 64.08
CV(%) 7.6% 4.3% 6.6% 9.1% 6% 7.4%

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%)
Sample 1 97 96 88 94 93 3.49 3.7%
Sample 2 212 195 228 218 213 11.98 5.6%
Sample 3 842 819 834 802 824 15.27 1.8%

*number of samples for each test n=16.

10ng/tube

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