Description
Boster’s human CA125 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CA125 has been precoated onto 96-well plates. Standards(Expression system for standard: Natural protein) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CA125 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CA125 amount of sample captured in plate.
Synonyms: CA125 | CA 125 | CA-125 | MUC16 | MUC 16 | MUC-16 | Mucin16 | Mucin 16 | Mucin-16 | Q8WXI7
Species: human CA125
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CA125
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: Natural protein
Gene name: Mucin-16 / mucin 16, cell surface associated
Protein name: Mucin-16
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|34|255|392|32|259|370
Standard deviation|1.36|20.14|17.64|1.47|21.75|21.09
CV(%)|4%|7.9%|4.5%|4.6%|8.4%|5.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 34 | 33 | 35 | 33 | 33 | 0.82 | 2.4% |
| Sample 2 | 255 | 252 | 237 | 261 | 251 | 8.84 | 3.5% |
| Sample 3 | 392 | 464 | 461 | 392 | 427 | 35.26 | 8.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1471-CAP|96-well plate precoated with anti- human CA125 antibody|1
EK1471-ST|Lyophilized recombinant human CA125 standard|1ng/tube×2
EK1471-DA|Biotinylated anti- human CA125 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
