Description
Boster’s human C-MET ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for C-MET has been precoated onto 96-well plates. Standards(NSO, E25-R307 (α) &S308-T932(β)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for C-MET is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human C-MET amount of sample captured in plate.
Synonyms: AUTS9|c Met|Hepatocyte growth factor receptor|HGF receptor|HGF/SF receptor|HGFR|MET|Met proto oncogene|Met proto oncogene tyrosine kinase|MET_HUMAN|Oncogene MET|Proto-oncogene c-Met|RCCP2|Scatter factor receptor|SF receptor|Tyrosine-protein kinase Met
Species: Human C-MET
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <5pg/ml Specifity: Natural and recombinant human C-MET
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, E25-R307(alpha)&S308-T932(beta)
Gene name: Hepatocyte growth factor receptor / met proto-oncogene
Protein name: Hepatocyte growth factor receptor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|210|601|1336|204|644|1384
Standard deviation|8.61|31.25|84.16|10.81|39.28|95.49
CV(%)|4.1%|5.2%|6.3%|5.3%|6.1%|6.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 210 | 196 | 181 | 199 | 196 | 10.35 | 5.2% |
| Sample 2 | 601 | 676 | 644 | 633 | 638 | 26.8 | 4.2% |
| Sample 3 | 1336 | 1534 | 1527 | 1377 | 1443 | 88.23 | 6.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0744-CAP|96-well plate precoated with anti- human C-MET antibody|1
EK0744-ST|Lyophilized recombinant human C-MET standard|10ng/tube×2
EK0744-DA|Biotinylated anti- human C-MET antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
