Description
Boster’s human AXL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for AXL has been precoated onto 96-well plates. Standards(NSO, E33-P440) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AXL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human AXL amount of sample captured in plate.
Synonyms: Adhesion related kinase|_x000D_AI323647|_x000D_Ark|_x000D__x000D__x000D_AXL oncogene|_x000D_AXL receptor tyrosine kinase|_x000D_AXL transforming gene|_x000D_AXL transforming sequence/gene|_x000D_JTK11|_x000D_Oncogene AXL|_x000D_Tyro7|_x000D_Tyrosine protein kinase receptor UFO|_x000D_UFO
Species: Human AXL
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human AXL
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, E33-P440
Gene name: Tyrosine-protein kinase receptor UFO / AXL receptor tyrosine kinase
Protein name: Tyrosine-protein kinase receptor UFO
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|103|593|1295|107|550|1194
Standard deviation|8.13|45.06|86.76|9.3|49.5|85.96
CV(%)|7.9%|7.6%|6.7%|8.7%|9%|7.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 103 | 88 | 85 | 85 | 90 | 7.46 | 8.2% |
| Sample 2 | 593 | 706 | 654 | 601 | 638 | 45.47 | 7.1% |
| Sample 3 | 1295 | 1325 | 1154 | 1246 | 1255 | 64.77 | 5.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0659-CAP|96-well plate precoated with anti- human AXL antibody|1
EK0659-ST|Lyophilized recombinant human AXL standard|10ng/tubex2
EK0659-DA|Biotinylated anti- human AXL antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
