Description
Boster’s human ACE ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ACE has been precoated onto 96-well plates. Standards(NSO, L30-L1261) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ACE amount of sample captured in plate.
Synonyms: ACE 1 | ACE T | Angiotensin converting enzyme | Angiotensin Converting Enzyme 1 | Angiotensinconverting enzyme | Angiotensin-converting enzyme | CD143 antigen | DCP 1 | DCP, DCP1 | Dipeptidyl carboxypeptidase 1 | Dipeptidyl carboxypeptidase I | Kininase II | MVCD3 | P12821 | Peptidyl dipeptidase A
Species: Human ACE
Assay: 0.78ng/ml-50ng/ml
Sensitivy: <5pg/ml Specifity: Natural and recombinant human ACE
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: NSO, L30-L1261
Gene name: Angiotensin-converting enzyme / angiotensin I converting enzyme
Protein name: Angiotensin-converting enzyme
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1278|6839|24157|1358|6350|25528
Standard deviation|62.62|465.05|1087.06|88.27|482.6|1225.34
CV(%)|4.9%|6.8%|4.5%|6.5%|7.6%|4.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1278 | 1310 | 1292 | 1425 | 1326 | 58.13 | 4.3% |
| Sample 2 | 6839 | 6994 | 7039 | 6669 | 6885 | 145.23 | 2.1% |
| Sample 3 | 24157 | 24884 | 22207 | 21600 | 23212 | 1350.66 | 5.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0557-CAP|96-well plate precoated with anti- human ACE antibody|1
EK0557-ST|Lyophilized recombinant human ACE standard|50ng/tubex2
EK0557-DA|Biotinylated anti- human ACE antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
