A cell-permeable aromatic amino-fluorescein derivative that can be oxidized and converted to fluorescein by ROS such as the hydroxyl radical

HPF – 1 mg

Brand:
Cayman
CAS:
359010-69-8
Storage:
-20
UN-No:
De Minimis - 1231 / 3

The biology of highly reactive oxygen radical species is of great interest in many biomedical research disciplines, including neurodegeneration, aging, cancer, and infectious diseases.{9477} There are a number of fluorescent reagents, such as 2,7-dichlorodihydrofluorescein (DCDHF), that can be used to detect free radicals, but they have significant limitations due to their facile oxidation by light and numerous non-radical oxidants such as hydrogen peroxide (H2O2).{12088} HPF is a cell-permeable aromatic amino-fluorescein derivative that has little intrinsic fluorescence.{11242} It undergoes oxidation only by highly reactive oxygen species (hROS) such as the hydroxyl radical, peroxynitrite, and hROS generated from a peroxidase/H2O2 system. It is inert to hypochlorite ion, nitric oxide, hydrogen peroxide (H2O2), superoxide, and other oxidants. Upon oxidation, HPF is converted to the highly fluorescent molecule fluorescein, with excitation/emission maxima of 490/515 nm, respectively, allowing the simple direct detection of highly reactive biological radicals.  

 

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Description

A cell-permeable aromatic amino-fluorescein derivative that can be oxidized and converted to fluorescein by ROS such as the hydroxyl radical, peroxynitrite, and ROS generated from a peroxidase/H2O2 system; ex/em = 490/515 nm

Formal name: (2-[6-(4′-hydroxy)phenoxy-3H-xanthene-3-on-9-yl]benzoic acid

Synonyms: 

Molecular weight: 424.4

CAS: 359010-69-8

Purity: ≥98%

Formulation: A solution in methyl acetate

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Product Type|Biochemicals|Labeling & Detection|Reactive Probes||Research Area|Oxidative Stress & Reactive Species|Reactive Nitrogen||Research Area|Oxidative Stress & Reactive Species|Reactive Oxygen

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