Description
Boster’s Equine GDF5 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDF5 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: A376-R495) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDF5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Equine GDF5 amount of sample captured in plate.
Synonyms: Growth and differentiation factor-5 ;Gdf5 ;gdf-5 ;
Species: Equine Gdf5
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity:
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: Expression system for standard: E.coli; Immunogen sequence: A376-R495
Gene name: Growth and differentiation factor-5 / growth differentiation factor 5
Protein name: Growth and differentiation factor-5
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 196 | 753 | 1345 | 221 | 834 | 1416 |
| Standard deviation | 10.97 | 51.2 | 99.53 | 16.57 | 69.22 | 121.77 |
| CV(%) | 6.8% | 6.8% | 7.4% | 7.5% | 8.3% | 8.6% |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 60 | 59 | 68 | 62 | 62 | 3.49 | 5.6% |
| Sample 2 | 323 | 317 | 316 | 281 | 309 | 16.52 | 5.3% |
| Sample 3 | 894 | 859 | 910 | 831 | 873 | 30.69 | 3.5% |
*number of samples for each test n=16.
10ng/tube
