Description
Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.{57056} It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.{57056,53978} Trimethylation of H3K36 is increased in the G1 and early S phases of the cell cycle where it binds to and recruits the mismatch recognition protein MutSα in preparation for DNA replication and mismatch repair.{46225} H3K36 trimethylation is found in greater amounts on exons compared with introns in C. elegans, mouse, and human genome-wide maps of histone H3 tail methylations.{46226} The loss of H3K36Me3 is correlated with poor prognosis in multiple cancer types while high levels increase expression of tumor suppressor genes.{56220} H3K36Me3 is negatively correlated with esophageal squamous cell carcinoma (ESCC) metastasis and positively correlated with increased survival in patients with ESCC. Cayman’s Histone H3K36Me3 Monoclonal Antibody can be used for chromatin immunoprecipitation (ChIP), ELISA, multiplex-based assay, and Western blot (WB) applications.
Synonyms: Histone H3 (Lys36me3)|Histone H3 (trimethyl K36)|Trimethyl Histone H3 (Lys36)|Trimethylated Histone 3 Lysine 36
Immunogen: A peptide corresponding to H3K36Me3
Formulation: 100 µg of protein A-affinity purified monoclonal antibody
Isotype: IgG
Applications: ChIP, ELISA, Multiplex-based Assays, WB
Origin:
Stability: 365 days
Application|ELISA||Application|Immunoprecipitation||Application|Multiplex||Application|Western Blot||Product Type|Antibodies|Monoclonal Antibodies||Research Area|Epigenetics, Transcription, & Translation|Erasers|Histone Demethylation||Research Area|Epigenetics, Transcription, & Translation|Histones/Histone Peptides|Methylated||Research Area|Epigenetics, Transcription, & Translation|Writers|Histone Methylation
