Description
His-tag Monoclonal Antibody is a probe for the immunochemical detection of histidine tags on recombinant proteins. Recombinant proteins are commonly labeled with affinity tags, such as 6-10 histidine residues (6x-10x His), to facilitate both their detection and purification.{59520} Poly-His tags are commonly utilized because of their small size, low potential to interfere in protein folding or activity, weak immunogenicity, and high affinity for transition metal ion matrices, such as Ni2+, used in immobilized metal-affinity chromatography (IMAC) for protein purification.{59521,59520} Cayman’s His-tag Monoclonal Antibody is comprised of rabbit IgG variable domains and the human IgG1 constant domain. This antibody can be used for ELISA and Western blot (WB) applications. It recognizes proteins containing 6x or 10x His tags fused to either the N- or C-terminus and is recognized by anti-human IgG secondary antibodies.
Synonyms: Deca-His Tag|Decahistidine Tag|Hexa-His Tag|Hexahistidine Tag|Poly-His Tag
Immunogen: Mixture of a peptide with a 6x His tag at the N-terminus and a peptide with a 6x His tag at the C-terminus
Formulation: 100 µg protein A-purified monoclonal antibody
Isotype: IgG
Applications: ELISA, WB
Origin:
Stability: 365 days
Application|ELISA||Application|Western Blot||Product Type|Antibodies|Monoclonal Antibodies
