Description
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an enzyme that catalyzes the conversion of glyceraldehyde-3-phosphate (Item No. 17865) to 1,3-bisphosphoglycerate during glycolysis and is involved in numerous additional cellular processes, including intracellular trafficking, receptor-mediated signaling, apoptosis, DNA repair, and the oxidative stress response.{54459,54460} It exists as a tetramer and is composed of an N-terminal domain, which contains binding sites for NAD+, phosphatidylserine, RNA, and glutathione, and a C-terminal catalytic domain.{54461} GAPDH is widely expressed and primarily localizes to the cytosol, where it has roles in glycolysis and intracellular trafficking.{54459,60077} It also localizes to the nucleus, mediating DNA integrity, gene transcription, and apoptosis, as well as to cellular membranes, where it has roles in membrane fusion and iron transport.{60077} GAPDH expression is increased by insulin, hypoxia-inducible factor-1 (HIF-1), p53, and nitric oxide (NO) and decreased by acetylated histones.{54459,54462} Aberrant mRNA and protein levels of GAPDH have been found in tumor biopsies from patients with a variety of cancers, including lung, renal cell, colorectal, or breast cancer.{60078} Cayman’s GAPDH (C-Term) Rabbit Monoclonal Antibody can be used for immunocytochemistry (ICC), immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and Western blot (WB) applications. The antibody recognizes the C-terminal region of GAPDH at approximately 36 kDa.
Synonyms: G3PD Protein, Human|GAPD Protein, Human|HEL-S-162eP Protein|Peptidyl-cysteine S-nitrosylase
Immunogen: Peptide from the C-terminal region of GAPDH
Formulation: 100 µl of protein A-affinity purified monoclonal antibody
Isotype: IgG
Applications: ICC, IP, ChIP, WB
Origin:
Stability: 365 days
Application|Immunocytochemistry||Application|Immunohistochemistry||Application|Immunoprecipitation||Application|Western Blot||Product Type|Antibodies|Monoclonal Antibodies||Research Area|Cell Biology|Cell Death|Apoptosis||Research Area|Cell Biology|Cell Signaling||Research Area|Cell Biology|Endomembrane System & Vesicular Trafficking||Research Area|Endocrinology & Metabolism|Carbohydrate Metabolism||Research Area|Epigenetics, Transcription, & Translation||Research Area|Oxidative Stress & Reactive Species|Reactive Sulfur|Glutathione
