An activated fluorescent molecule (excitation: 494 nm

Fluorescein-5-maleimide – 5 mg

Brand:
Cayman
CAS:
75350-46-8
Storage:
-20
UN-No:
Non-Hazardous - /

Fluorescein-5-maleimide is an activated fluorescent molecule (excitation: 494 nm, emission: 519 nm) that is used to easily conjugate fluorescein to proteins.{26643,26644,26645} The maleimide group reacts optimally with sulfhydryl groups on cysteine side chains at pH 7, forming a stable thioether bond.{26642} The compound can be used to label proteins for the detection of conformational changes, assembly of multisubunit complexes, and ligand-binding processes.{26646}  

 

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Description

An activated fluorescent molecule (excitation: 494 nm, emission: 519 nm) used for labeling proteins; reacts optimally with sulfhydryl groups on cysteine side chains at pH 7, forming a stable thioether bond

Formal name: 1-(3′,6′-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthen]-5-yl)-1H-pyrrole-2,5-dione

Synonyms:  5-Maleimido-fluorescein|5-MF

Molecular weight: 427.4

CAS: 75350-46-8

Purity: ≥90%

Formulation: An orange to brown solid

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Dyes, Stains, & Indicators||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area

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