A cell-permeable fluorescent calcium indicator; cleaved by intracellular esterases to release Fluo-4

Fluo-4 AM – 1 mg

Brand:
Cayman
CAS:
273221-67-3
Storage:
-20
UN-No:
Non-Hazardous - /

Fluo-4 acetoxymethyl ester (Fluo-4 AM) is a cell-permeable fluorescent calcium indicator.{57160} It is cleaved by intracellular esterases to release Fluo-4, which binds to calcium with a Kd value of 345 nM and displays excitation/emission maxima of 490/520 nm, respectively. Fluo-4 AM has been used to measure intracellular calcium levels in live cells by fluorescent microscopy or flow cytometry.{57160,57161}  

 

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SKU: 31143 - 1 mg Category:

Description

A cell-permeable fluorescent calcium indicator; cleaved by intracellular esterases to release Fluo-4, which binds to calcium (Kd = 345 nM); ex/em = 490/520 nm, respectively; has been used to measure intracellular calcium levels in live cells by fluorescent microscopy or flow cytometry

Formal name: N-[4-[6-[(acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-glycine (acetyloxy)methyl ester

Synonyms:  Fluo-4 acetoxymethyl ester

Molecular weight: 1,096.90

CAS: 273221-67-3

Purity: ≥95%

Formulation: A solid

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