A cell-permeable AM ester of the fluorescent calcium indicator fluo-3

Fluo-3 AM – 500 µg

Brand:
Cayman
CAS:
121714-22-5
Storage:
-20
UN-No:
Non-Hazardous - 1648 / 3

Fluo-3 is a fluorescent calcium indicator commonly used in flow cytometry (FC) and cell-based experiments to detect changes in intracellular calcium levels.{23813} Its absorption maximum at 506 nm makes it compatible with excitation at 488 nm by argon-ion laser sources. Fluo-3 provides intense fluorescence upon binding calcium, detected at a maximum emission at 526 nm which can be monitored by FL1 (green, 525 nm band pass) sensors in flow cytometry. Fluo-3 AM is an acetoxymethyl (AM) ester of fluo-3. This form, unlike fluo-3, is cell permeable and readily taken up by living cells, whereupon the AM moiety is enzymatically removed to produce fluo-3.{22804}  

 

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Description

A cell-permeable AM ester of the fluorescent calcium indicator fluo-3

Formal name: N-[4-[6-[(acetyloxy)methoxy]-2,7-dichloro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-glycine, (acetyloxy)methyl ester

Synonyms:  Fluo-3 Acetoxymethyl ester

Molecular weight: 1,129.90

CAS: 121714-22-5

Purity: ≥90%

Formulation: A solution in acetonitrile

Application|Flow Cytometry||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Dyes, Stains, & Indicators||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Cell Biology|Cell Signaling|Calcium Mobilization

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