A pH-dependent fluorescent probe to label dopaminergic cells; excitation spectra of 340 and 370 nm at pH 5 and 7.4

FFN-102 (trifluoroacetate salt) – 10 mg

Brand:
Cayman
CAS:
1234064-11-9
Storage:
-20
UN-No:
Non-Hazardous - /

FFN-102 is a fluorescent false neurotransmitter (FFN) that is a substrate for the dopamine transporter (DAT) and vesicular monoamine transporter 2 (VMAT2).{38320} It is a pH-dependent fluorescent probe that labels dopamine cell bodies, axons, and presynaptic terminals. It can also be used to monitor dopamine exocytosis.{38319} It has a pKa of 6.2 and displays pH-dependent excitation spectra of 340 and 370 nm at pH 5 and 7.4, respectively, which correspond to vesicular and cytoplasmic pH values.{38320} The emission spectrum of FFN-102 is pH-independent at 453 nm, but the intensity of emission is pH-dependent with a higher intensity at a pH of 7.4. FFN-102 inhibits DAT (13.6% at a concentration of 10 µM) and the serotonin (5-HT) receptor subtype 5-HT2c (Ki = ~3 µM) but does not bind to 37 other central nervous system receptors and transporters, including dopamine receptors, up to a concentration of 10 µM.{38320}  

 

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Description

A pH-dependent fluorescent probe to label dopaminergic cells; excitation spectra of 340 and 370 nm at pH 5 and 7.4, respectively, which correspond to vesicular and cytoplasmic pH values; emission spectrum of 453 nm, with a higher intensity at pH 7.4; inhibits DAT (13.6% at a concentration of 10 µM) and the 5-HT receptor subtype 5-HT2c (Ki = ~3 µM); does not bind to 37 central nervous system receptors, including dopamine and serotonin receptors, up to a concentration of 10 µM

Formal name: 4-(2-aminoethyl)-6-chloro-7-hydroxy-2H-1-benzopyran-2-one 2,2,2-trifluoroacetate

Synonyms: 

Molecular weight: 353.7

CAS: 1234064-11-9

Purity: ≥98%

Formulation: A crystalline solid

Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Neuroscience

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