A SHAPE reagent; forms stable 2’-hydroxy adducts with RNA

FAI – 5 mg

Brand:
Cayman
CAS:
1415238-77-5
Storage:
-20
UN-No:
Non-Hazardous - /

FAI is a selective hydroxyl acylation analyzed by primer extension (SHAPE) electrophile reagent used to map RNA structures in vivo by forming stable 2’-hydroxy adducts with RNA, blocking reverse transcriptase elongation.{42234,42235} Its activity can be quenched by dithiothreitol (DTT) in vitro and in living cells.{42235} FAI has been used to probe the secondary structure of mouse embryonic stem cell 5S rRNA in vitro.{42234}  

 

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SKU: 23429 - 5 mg Category:

Description

A SHAPE reagent; forms stable 2’-hydroxy adducts with RNA, blocking reverse transcription elongation; activity can be quenched by DTT in vitro and in vivo; has been used to probe the secondary structure of mouse embryonic stem cell 5S rRNA in vitro

Formal name: 1H-imidazol-1-yl(2-methyl-3-furanyl)-methanone

Synonyms:  5S rRNA Modificator|Furoyl Acylimidazole

Molecular weight: 176.2

CAS: 1415238-77-5

Purity: ≥95%

Formulation: A crystalline solid

Product Type|Biochemicals|Labeling & Detection||Product Type|Biochemicals|Small Molecule Inhibitors|Nucleic Acid Turnover/Signaling||Research Area|Cell Biology|Stem Cell Research||Research Area|Epigenetics, Transcription, & Translation|RNA, DNA, & Protein Synthesis

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