Description
Boster’s Canine Neurotrophin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Neurotrophin-3 has been precoated onto 96-well plates. Standards(sf21, Y139-T257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Canine Neurotrophin-3 amount of sample captured in plate.
Synonyms: Neurotrophin-3;NT-3;HDNF;Nerve growth factor 2;NGF-2;Neurotrophic factor;Ntf3;Ntf-3;
Species: Canine Ntf3
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <2pg/ml Specifity:
Cross Reactivity: There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine. Antibodies: monoclonal antibody from rat|polyclonal antibody from goat
Anticoagulant:
Immunogen: Expression system for standard: sf21; Immunogen sequence: Y139-T257
Gene name: Neurotrophin-3 / Neurotrophin-3
Protein name: Neurotrophin-3
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 88 | 329 | 682 | 113 | 395 | 753 |
| Standard deviation | 4.84 | 19.74 | 30.01 | 7.12 | 28.44 | 39.16 |
| CV(%) | 5.5 | 6 | 4.4 | 7.2 | 7.2 | 5.2 |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 31 | 31 | 33 | 29 | 31 | 1.41 | 4.5% |
| Sample 2 | 195 | 189 | 201 | 195 | 195 | 4.24 | 2.1% |
| Sample 3 | 532 | 466 | 458 | 478 | 483 | 28.89 | 5.9% |
*number of samples for each test n=16.
10ng/tube
