Antigen: DNMT3a expressed in bacteria The epitope was found to be located near the C-terminus (amino acids 705-908) • Host: mouse

DNA Methyltransferase 3a Monoclonal Antibody (Clone 64B1446) – 1 ea

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Cayman
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Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, three families of mammalian DNA methyltransferase genes have been identified which include DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The DNMT3 family members, DNMT3a and DNMT3b, are strongly expressed in ES cells but their expression is down regulated in differentiating embyonic stem (ES) cells and is low in adult somatic tissue. It has been shown that naturally occurring mutations of DNMT3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.  

 

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SKU: 13484 - 1 ea Category:

Description

Antigen: DNMT3a expressed in bacteria The epitope was found to be located near the C-terminus (amino acids 705-908) • Host: mouse, clone 64B1446 • Isotype: IgG1κ • Cross Reactivity: (+) human and mouse DNMT3a • Application(s): ChIP, IF/ICC, IHC (paraffin), and WB • The DNMT3 family members DNMT3a and DNMT3b are strongly expressed in embryonic stem cells. Their expression is down regulated in differentiating cells and low in adult sematic tissue.

Synonyms: DNMT3a

Immunogen: recombinant mouse Dnmt3a expressed in bacteria. The epitope was found to be located near the C-terminus (amino acids 705-908)

Formulation: 100 µg of protein G-purified IgG in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide

Isotype: IgG1κ

Applications: WB, IF/ICC, ChIP, and IHC (paraffin)

Origin: Animal/Mouse

Stability: 365 days

Application|Immunocytochemistry||Application|Immunofluorescence||Application|Immunohistochemistry||Application|Western Blot||Product Type|Antibodies|Monoclonal Antibodies||Research Area|Epigenetics, Transcription, & Translation|Writers|DNA/RNA Methylation

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