A steroid-based cationic lipid; forms a complex with plasmid DNA and decreases plasmid DNA migration in an agarose-gel retardant assay at charge ratio ≥4; facilitates transfection of plasmid DNA into H1299 and HeLa cells; has been used

Dios-Arg (trifluoroacetate salt) – 1 mg

Brand:
Cayman
CAS:
1807353-31-6
Storage:
-20
UN-No:
Excepted Quantity - 1170 / 3

Dios-Arg is a steroid-based cationic lipid that contains a diosgenin skeleton coupled to an L-arginine head group.{47130} It forms a complex with plasmid DNA and decreases plasmid DNA migration in an agarose-gel retardant assay at charge ratio greater than or equal to 4.{44005} Dios-Arg facilitates transfection of plasmid DNA into H1299 and HeLa cells in the presence and absence of fetal bovine serum, an effect that is reversed by the lipid raft-mediated endocytosis inhibitor methyl-β-cyclodextrin (Item No. 21633) and the caveolae-mediated endocytosis inhibitor genistein (Item No. 10005167). It has been used, coupled to 1,2-dioleoyl-sn-glycero-3-PE (DOPE; Item No. 15091), to bind siRNA and plasmid DNA to form cationic lipid nanoparticles for intracellular transport. Dios-Arg is cytotoxic to H1299 and HeLa cells (IC50s = 83.5 and 74.1 µg/ml, respectively).{44005}  

 

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SKU: 25945 - 1 mg Category:

Description

A steroid-based cationic lipid; forms a complex with plasmid DNA and decreases plasmid DNA migration in an agarose-gel retardant assay at charge ratio ≥4; facilitates transfection of plasmid DNA into H1299 and HeLa cells; has been used, coupled to DOPE, to bind siRNA and plasmid DNA to form cationic lipid nanoparticles for intracellular transport

Formal name: (3β,25R)-3-[6-[[(2S)-2-amino-5-[(aminoiminomethyl)amino]-1-oxopentyl]amino]hexanoate] spirost-5-en-3-ol, bis(2,2,2-trifluoroacetate)

Synonyms: 

Molecular weight: 912

CAS: 1807353-31-6

Purity: ≥95%

Formulation: A solution in ethanol

Product Type|Biochemicals|Lipids|Sterol Lipids||Research Area|Cell Biology||Research Area|Lipid Biochemistry|Sterol Lipids

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