A biotinylated form of DEPMPO

DEPMPO-biotin – 100 µg

Brand:
Cayman
CAS:
936224-52-1
Storage:
-80
UN-No:
De Minimis - 1170 / 3

DEPMPO is a nitrone that is used to spin trap reactive O-, N-, S-, and C-centered radicals and allow their characterization when used in association with electron spin resonance. It is noted for the stability of adducts formed.{17433} DEPMPO can be used in vitro or in vivo, as it crosses lipid bilayer membranes and is a good trapping agent in biological systems.{17434,17435} DEPMPO-biotin is a biotinylated form of DEPMPO which retains the outstanding persistency of its adducts.{17436} The biotin moiety offers an effective means for monitoring biodistribution in cells, tissues, and organs when used with an avidin-conjugated reporter. Importantly, DEPMPO-biotin binds free radicals, such as S-nitroso groups, on proteins, producing adducts that can be analyzed via the biotin tag. This direct labeling of S-nitrosothiols (SNO) thus serves as an effective alternative to the more cumbersome biotin-switch method for monitoring SNO formation.  

 

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Description

A biotinylated form of DEPMPO, which is used to spin trap reactive O-, N-, S-, and C-centered radicals; offers monitored biodistribution in cells, tissues and organs when used with an avidin-conjugated reporter; binds free radicals on proteins, producing adducts that can be analyzed via the biotin tag.

Formal name: N-[3-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]propyl]-[(2S,3R)-2-(diethoxyphosphinyl)-3,4-dihydro-2-methyl-1-oxido-2H-pyrrol-3-yl]methyl ester-carbamic acid

Synonyms: 

Molecular weight: 591.7

CAS: 936224-52-1

Purity: ≥95%

Formulation: A solution in ethanol

Product Type|Biochemicals|Ox Stress Reagents|Spin Traps||Research Area|Oxidative Stress & Reactive Species|Reactive Nitrogen||Research Area|Oxidative Stress & Reactive Species|Reactive Oxygen|Non-enzymatic||Research Area|Oxidative Stress & Reactive Species|Reactive Sulfur

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