A cell-permeable

DAF-FM diacetate – 1 mg

Brand:
Cayman
CAS:
254109-22-3
Storage:
-20
UN-No:
Non-Hazardous - /

DAF-FM diacetate is a cell-permeable, fluorescent probe for the detection and bioimaging of nitric oxide (NO) with excitation/emission maxima of 495/515 nm. It passively diffuses across cellular membranes and, once inside cells, is deacetylated by intracellular esterases to become DAF-FM. The fluorescence quantum yield of DAF-FM is ~0.005, but increases about 160-fold, to ~0.81, after reacting with NO.{30226} DAF-FM is advantageous over the NO probe, DAF-2 (Item No. 85160) for several reasons: 1) the spectra of the NO adduct of DAF-FM are independent of pH above pH 5.5; 2) the NO adduct of DAF-FM is significantly more photostable than that of DAF-2; 3) the NO detection limit of DAF-FM (~3 nM) is more sensitive than that of DAF-2 (~5 nM).{30226,30225}  

 

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Description

A cell-permeable, fluorescent probe for the detection and bioimaging of NO (ex. max 495 nm/em. max 515 nm)

Formal name: 3′,6′-bis(acetyloxy)-4-amino-2′,7′-difluoro-5-(methylamino)-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one

Synonyms:  4-Amino-5-methylamino-2′,7′-difluorofluorescein diacetate|DAF-FM DA

Molecular weight: 496.4

CAS: 254109-22-3

Purity: ≥98%

Formulation: A solid

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Oxidative Stress & Reactive Species|Reactive Nitrogen

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