A fluorescent probe that competitively binds HDAC; demonstrates fluorescence excitation and emission maxima of 325 and 400 nm

coumarin-SAHA – 25 mg

Brand:
Cayman
CAS:
1260635-77-5
Storage:
-20
UN-No:
Non-Hazardous - /

Suberoylanilide hydroxamic acid (SAHA) is a class I and class II histone deacetylase (HDAC) inhibitor that binds directly to the catalytic site of the enzyme thereby blocking substrate access.{14739} coumarin-Suberoylanilide hydroxamic acid (c-SAHA) is a SAHA derivative where the anilino “cap” group is replaced by 7-amino-4-methylcoumarin to produce a fluorescent probe that competitively binds HDAC. {18767} The fluorescence excitation and emission maxima of free c-SAHA is 325 and 400 nm, respectively and is quenched by 50% when bound to HDAC8.{18767} This probe can be used to determine binding affinities and dissociation off-rates of HDAC enzyme-inhibitor complexes and is well-suited for high-throughput screening.  

 

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SKU: 10671 - 25 mg Category:

Description

A fluorescent probe that competitively binds HDAC; demonstrates fluorescence excitation and emission maxima of 325 and 400 nm, respectively, which is quenched by 50% when bound to HDAC

Formal name: N1-hydroxy-N8-(4-methyl-2-oxo-2H-chromen-7-yl)-octanediamide

Synonyms:  coumarin-Suberoylanilide Hydroxamic Acid

Molecular weight: 346.4

CAS: 1260635-77-5

Purity: ≥98%

Formulation: A crystalline solid

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Product Type|Biochemicals|Small Molecule Inhibitors|Deacetylases||Research Area|Epigenetics, Transcription, & Translation|Erasers|Histone Deacetylation

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