Description
Cofactor of BRCA1 (COBRA1), also known as negative elongation factor B (NELFB), is a 580-amino acid nuclear protein encoded by NELFB in humans.{46946,46943} It induces chromosome decondensation by targeting lac operator-containing chromosome regions and is a component of NELF, a four-subunit complex that suppresses RNA polymerase II elongation in vitro. COBRA1 protein levels are enriched in mammary gland luminal epithelium, and it has a role in modulating estrogen-dependent and -independent expression of oncogenes in various breast cancer cell lines.{46943} Ectopic expression or knockdown of NELFB suppresses or accelerates breast cancer cell growth, respectively, as well as enhances androgen-mediated transcription.{46943,46944} COBRA1 also interacts with the activated protein-1 (AP-1) family members c-Jun and c-Fos, and inhibits AP-1-mediated transcription in vitro.{46945} Cayman’s COBRA1 Monoclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes COBRA1 at approximately 62 kDa from human, mouse, and rat samples.
Synonyms: Cofactor of BRCA1|Negative Elongation Factor B|Negative Elongation Factor Complex Member B|Negative Elongation Factor Protein B|NELF-B
Immunogen: Recombinant human full-length COBRA1 expressed in E. coli
Formulation: 100 µl of protein G-purified mouse monoclonal antibody
Isotype:
Applications: WB
Origin:
Stability: 365 days
Application|Western Blot||Product Type|Antibodies|Monoclonal Antibodies||Research Area|Cancer|Tumor Microenvironment||Research Area|Epigenetics, Transcription, & Translation|RNA, DNA, & Protein Synthesis||Research Area|Epigenetics, Transcription, & Translation|Transcription Factors
