A highly sensitive

Citrulline-specific Probe-rhodamine – 250 µg

Brand:
Cayman
CAS:
2309313-01-5
Storage:
-20
UN-No:
Non-Hazardous - /

Protein arginine deiminases (PADs) catalyze the posttranslational modification of arginine residues on proteins to form citrulline, which plays a large role in regulating gene expression.{18134} Abnormally high PAD activity has been observed in a host of human diseases.{18134,18137} Citrulline-specific probe-rhodamine is a highly sensitive, rhodamine phenylglyoxal-based fluorophore that specifically detects protein citrullination via a chemoselective reaction between glyoxal and citrulline.{26196} This chemical probe (comprised of a single isomer) is capable of reacting with any citrulline-containing protein and can be analyzed with fluorescent imaging (excitation 532 nm; emission 580 nm).{26196} When added at 100 µM for 30 minutes at acidic pH, this probe has a reported limit of detection of ~10 ng for citrullinated histone H3 and ~1 ng for autodeiminated PAD4.{26196}  

 

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Description

A highly sensitive, rhodamine phenylglyoxal-based fluorophore that specifically detects protein citrullination via a chemoselective reaction between glyoxal and citrulline; reacts with any citrulline-containing protein and can be analyzed with fluorescent imaging (Ex. 532 nm; Em. 580 nm); limit of detection of ~10 ng for citrullinated histone H3 and ~1 ng for autodeiminated PAD4 when applied at 100 µM for 30 minutes at acidic pH

Formal name: 3′,6′-bis(dimethylamino)-3-oxo-N-((1-(1-oxo-1-((3-(2-oxoacetyl)phenyl)amino)propan-2-yl)-1H-1,2,3-triazol-4-yl)methyl)-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxamide, monohydrate

Synonyms:  Rh-PG|Rhodamine Phenylglyoxal

Molecular weight: 731.8

CAS: 2309313-01-5

Purity: ≥90%

Formulation: A lyophilized solid

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Epigenetics, Transcription, & Translation|Writers|Citrullination

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