A steroid-based cationic lipid; forms a complex with plasmid DNA and decreases plasmid DNA migration in an agarose-gel retardant assay; facilitates transfection of plasmid DNA into H1299 and HeLa cells in the presence or absence of fetal bovine serum; cytotoxic to H1299 cells (IC50 = 88.5 μg/ml)

Cho-Arg (trifluoroacetate salt) – 1 mg

Brand:
Cayman
CAS:
1609010-56-1
Storage:
-20
UN-No:
Non-Hazardous - 1170 / 3

Cho-Arg is a steroid-based cationic lipid that contains a cholesterol skeleton coupled to an L-arginine head group.{44005} It forms a complex with plasmid DNA and decreases plasmid DNA migration in an agarose-gel retardant assay at charge ratios greater than or equal to 4. Cho-Arg facilitates transfection of plasmid DNA into H1299 and HeLa cells in the presence or absence of fetal bovine serum, an effect that is reversed by the lipid raft-mediated endocytosis inhibitor methyl-β-cyclodextrin (Item No. 21633) and the caveolae-mediated endocytosis inhibitor genistein (Item No. 10005167). It is cytotoxic to H1299 cells (IC50 = 88.5 μg/ml).  

 

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SKU: 25943 - 1 mg Category:

Description

A steroid-based cationic lipid; forms a complex with plasmid DNA and decreases plasmid DNA migration in an agarose-gel retardant assay; facilitates transfection of plasmid DNA into H1299 and HeLa cells in the presence or absence of fetal bovine serum; cytotoxic to H1299 cells (IC50 = 88.5 μg/ml)

Formal name: cholest-5-en-3-ol (3β)-3-[6-[[(2S)-2-amino-5-[(aminoiminomethyl)amino]-1-oxopentyl]amino]hexanoate], 2,2,2-trifluoroacetate (1:2)

Synonyms: 

Molecular weight: 884.1

CAS: 1609010-56-1

Purity: ≥95%

Formulation: A solution in ethanol

Product Type|Biochemicals|Lipids|Sterol Lipids||Research Area|Cell Biology||Research Area|Lipid Biochemistry|Sterol Lipids

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