A fluorescent cleavage product of CFDA-SE; is less cell-permeable than CFDA-SE; ex/em = 491/518 nm; can be retained in cells for at least eight weeks; fluorescence dilution can be used to analyze cell proliferation; fluorescence can be used to track cell migration in vivo

CFSE – 5 mg

Brand:
Cayman
CAS:
92557-80-7
Storage:
-20
UN-No:
Non-Hazardous - /

CFSE is a fluorescent product of CFDA-SE (Item No. 14456) cleavage by intracellular esterases.{36590} CFSE lacks the diacetate groups of CFDA-SE and, as a result, is less cell permeable. CFSE fluorescence is cytoplasmic, and it has excitation/emission maxima of 491 and 518 nm, respectively.{36591} It covalently couples to intracellular molecules via its succinimidyl group and can be retained within cells for at least eight weeks. The dilution of CFSE fluorescence resulting from cell division can be used to analyze cell proliferation.{17820} Its fluorescence can also be used to track cell migration in vivo.{17802}  

 

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Description

A fluorescent cleavage product of CFDA-SE; is less cell-permeable than CFDA-SE; ex/em = 491/518 nm; can be retained in cells for at least eight weeks; fluorescence dilution can be used to analyze cell proliferation; fluorescence can be used to track cell migration in vivo

Formal name: 3′,6′-dihydroxy-3-oxo-spiro[isobenzofuran-1(3H),9′-[9H]xanthene]-5-carboxylic acid, 2,5-dioxo-1-pyrrolidinyl ester

Synonyms:  5-Carboxyfluorescein NHS ester|5-Carboxyfluorescein N-Succinimidyl ester|5-Carboxyfluorescein-N-hydroxysuccinimide ester|5-FAM N-succinimidyl ester|5-FAM SE

Molecular weight: 473.4

CAS: 92557-80-7

Purity: ≥95%

Formulation: A crystalline solid

Application|Flow Cytometry||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area

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