A stable

CFDA-SE – 50 mg

Brand:
Cayman
CAS:
150347-59-4
Storage:
-20
UN-No:
Non-Hazardous - /

CFDA-SE is a stable, cell-permeable dye that consists of a fluorescein molecule containing two acetate moieties and a succinimidyl ester (SE) functional group.{17820} Upon diffusion into the intracellular environment, the acetate groups are cleaved by cellular esterases leaving CFSE (Item No. 16802), which is fluorescent and not cell permeable.{36590} CFSE covalently couples to intracellular molecules via its succinimidyl group and can be retained in cells for at least eight weeks.{36591} CFDA-SE is often used to assay cell proliferation as it is partitioned with high fidelity between daughter cells for up to eight generational divisions.{17820,17802,21243} CFSE, the cleavage product of CFDA-SE, displays excitation/emission maxima of 491/518 nm, respectively.{36591}  

 

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Description

A stable, cell-permeable dye employed to assay cell proliferation as it is partitioned with high fidelity between daughter cells for up to eight generational divisions; CFSE, the intracellular cleavage product, has ex/em = 491/518 nm

Formal name: 3′,6′-bis(acetyloxy)-3-oxo-2,5-dioxo-1-pyrrolidinyl ester-spiro[isobenzofuran-1(3H),9′-[9H]xanthene]-ar-carboxylic acid

Synonyms:  5(6)-Carboxyfluorescein diacetate succinimidyl ester|5(6)-CFDA N-succinmidyl ester

Molecular weight: 557.5

CAS: 150347-59-4

Purity: ≥95% (mixture of regioisomers)

Formulation: A crystalline solid

Application|Flow Cytometry||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Cell Biology

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