A cell-permeable dye that is cleaved by intracellular esterases

Calcein AM – 1 mg

Brand:
Cayman
CAS:
148504-34-1
Storage:
-20
UN-No:
Non-Hazardous - /

Calcein AM is a cell-permeable dye that, upon entering live cells, is cleaved by intracellular esterases, leaving membrane-impermeant calcein (Item No. 16221), a fluorescent indicator with absorption and emission maxima of 494 and 517 nm, respectively. It can be used as an indicator of cell viability, cell-cell communication, cytotoxicity, or changes in intracellular calcium, fluoride, iron, or mercury.{16310,22804} As calcein is a substrate for multidrug resistance-associated protein 1 (MRP1) and multidrug resistance protein 3 (MDR3, P-glycoprotein 3), calcein AM is used to study these proteins and their regulation.{19233,23971,30807}  

 

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Description

A cell-permeable dye that is cleaved by intracellular esterases, leaving membrane-impermeant calcein, a fluorescent indicator with absorption and emission maxima of 494 and 517 nm, respectively

Formal name: N,N’-[[3’,6’-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9’-[9H]xanthene]-2’,7’-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]]-glycine, 1,1’-bis[(acetyloxy)methyl] ester

Synonyms:  Calcein Acetoxymethyl ester|NSC 689290

Molecular weight: 994.9

CAS: 148504-34-1

Purity: ≥90%

Formulation: A crystalline solid

Application|Flow Cytometry||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Cell Biology|Cell Signaling|Calcium Mobilization

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