A fluorescent ceramide analog that contains a saturated bond in the C-4/C-5 position of the sphingosine backbone

C12 NBD dihydro Ceramide (d18:0/12:0) – 1 mg

Brand:
Cayman
CAS:
474943-05-0
Storage:
-20
UN-No:
De Minimis - 1992 / 3|6.1

Ceramidase catalyzes the hydrolysis of the N-acyl linkage between the fatty acid and sphingosine base in ceramide to produce sphingosine and a free fatty acid. Three isoforms of ceramidases (acid, neutral, and alkaline) have been characterized based on differences in their catalytic pH optimum.{9268,10050} C12 NBD dihydro ceramide is a fluorescent ceramide analog that contains a saturated bond in the C-4/C-5 position of the sphingosine backbone. C-12 NBD ceramide, which contains the C-4/C-5 double bond, is a fluorescent ceramidase substrate that can be used for the measurement of alkaline and neutral ceramidase activity from a variety of sources.{14063} C12 NBD dihydro ceramide may exhibit reduced activity in ceramidase assays compared to C-12 NBD ceramide. This is based on the observation that saturation of the C-4/C-5 sphingosine bond in C-16-ceramide results in approximately a 10-fold reduction in efficiency as a substrate for rat brain ceramidase compared to the unsaturated substrate.{14071} However, experimental characterization of C12 NBD dihydro ceramide as a ceramidase substrate needs to be performed.  

 

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SKU: 10008097 - 1 mg Category:

Description

A fluorescent ceramide analog that contains a saturated bond in the C-4/C-5 position of the sphingosine backbone

Formal name: N-[(1S,2R)-2-hydroxy-1-(hydroxymethyl)heptadecyl]-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-dodecanamide

Synonyms:  NBD Cer(d18:0/12:0)|NBD Ceramide (d18:0/12:0)

Molecular weight: 661.9

CAS: 474943-05-0

Purity: ≥98%

Formulation: A solution in chloroform:ethanol (1:1)

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Product Type|Biochemicals|Lipids|Sphingolipids||Research Area|Lipid Biochemistry|Sphingolipids

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