Bovine CXCL14 PicoKine™ ELISA Kit

Brand:
Boster Bio
Storage:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Bovine CXCL14. 96wells/kit, with removable strips.   

 

Available on backorder

SKU: EK1285-BV Category:

Description

Boster’s Bovine CXCL14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL14 has been precoated onto 96-well plates. Standards(E.coli, S35-E111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Bovine CXCL14 amount of sample captured in plate.


Synonyms: C-X-C motif chemokine 14;Chemokine BRAK;MIP-2G;Small-inducible cytokine B14;CXCL14;MIP2G, NJAC, SCYB14;PSEC0212, UNQ240/PRO273;

Species: Bovine CXCL14

Assay: 62.5pg/ml-4000pg/ml

Sensitivy: <10pg/ml Specifity:

Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat

Anticoagulant: heparin or EDTA

Immunogen: Expression system for standard: E.coli; Immunogen sequence: S35-E111

Gene name: C-X-C motif chemokine 14 / C-X-C motif chemokine 14

Protein name: C-X-C motif chemokine 14

Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)


Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 16 16 16 24 24 24
Mean(pg/ml) 987 1638 3285 1387 2694 3525
Standard Deviation 35.5 68.8 187.2 56.87 156 224.2
CV(%) 3.6 4.2 5.7 4.1 5.8 6.4

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%)
Sample 1 174 182 174 200 182 10.61 5.8%
Sample 2 718 818 718 802 764 46.34 6%
Sample 3 1106 1003 1075 1062 1061 37.36 3.5%

*number of samples for each test n=16.


10ng/tube