A stable precursor to the fluorescent ALDH substrate BAAA; converted under acidic conditions to BAAA

BODIPY-aminoacetaldehyde diethyl acetal – 1 mg

Brand:
Cayman
CAS:
247069-93-8
Storage:
-20
UN-No:
De Minimis - 1231 / 3

BODIPY-aminoacetaldehyde diethyl acetal (BAAA-DA) is a stable precursor to BODIPY-aminoacetaldehyde, a cell-permeable fluorescent substrate for aldehyde dehydrogenase (ALDH).{28080,28081} BODIPY-aminoacetaldehyde diethyl acetal is converted under acidic conditions to BODIPY-aminoacetaldehyde (BAAA).{28081} BAAA is cell-permeant and is converted intracellularly by ALDH to BODIPY aminoacetate (BAA), which is retained by cells and can be used to identify cells with high ALDH activity.{28080} BAA is a substrate for the efflux pump P-glycoprotein (P-gp) but co-application of BAAA with a P-gp inhibitor, such as verapamil (Item No. 14288), inhibits BAA efflux.{28081} BAAA-DA has been used to isolate human hematopoietic progenitor cells, which have high ALDH activity, and via flow cytometry to sort cancer stem cells that contain high levels of ALDH.{28080,49664} BAA used in cells can be excited at 488 nm and displays an emission maximum of 512 nm.{49665}  

 

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SKU: 9002056 - 1 mg Category:

Description

A stable precursor to the fluorescent ALDH substrate BAAA; converted under acidic conditions to BAAA, which is cell-permeant and is converted intracellularly to BAA, which can be used to identify cells with high ALDH activity; co-application of BAAA with a P-gp inhibitor inhibits efflux of BAA; has been used to isolate human hematopoietic progenitor cells and via flow cytometry to sort cancer stem cells that contain high levels of ALDH; can be excited at 488 nm and displays and emission maximum of 512 nm

Formal name: (T-4)-[N-(2,2-diethoxyethyl)-5-[(3,5-dimethyl-2H-pyrrol-2-ylidene-kN)methyl]-1H-pyrrole-2-propanamidato-κN1]difluoro-boron

Synonyms:  BAAA-DA

Molecular weight: 407.3

CAS: 247069-93-8

Purity: ≥95%

Formulation: A solution in methyl acetate

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