A cell-permeable inhibitor of non-muscle myosin II ATPases; inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (IC50s = 0.5-5 µM); poorly inhibits smooth muscle myosin (IC50 = 80 µM); (S)-nitro-blebbistatin is a more stable form of (-)-Blebbistatin

(–)-Blebbistatin – 5 mg

Brand:
Cayman
CAS:
856925-71-8
Storage:
-20
UN-No:
Non-Hazardous - /

(–)-Blebbistatin is a selective cell-permeable inhibitor of non-muscle myosin II ATPases and the active enantiomer of (+)-blebbistatin (Item No. 13165).{16452,17037} It rapidly and reversibly inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (IC50s = 0.5-5 µM), while poorly inhibiting smooth muscle myosin (IC50 = 80 µM).{17036} Through these effects, it blocks apoptosis-related bleb formation, directed cell migration, and cytokinesis in vertebrate cells. However, prolonged exposure to blue light (450-490 nm) results in degradation of blebbistatin to an inactive product via cytotoxic intermediates, which may be problematic for its use in fluorescent live cell imaging applications.{17038,24037} (S)-nitro-Blebbistatin (Item No. 13186) is a more stable form of (–)-blebbistatin.{16725} The addition of a nitro group stabilizes the molecule to circumvent its degradation by prolonged blue light exposure. (S)-nitro-Blebbistatin has the same stereochemistry as the active (–)-blebbistatin enantiomer.  

 

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SKU: 13013 - 5 mg Category:

Description

A cell-permeable inhibitor of non-muscle myosin II ATPases; inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (IC50s = 0.5-5 µM); poorly inhibits smooth muscle myosin (IC50 = 80 µM); (S)-nitro-blebbistatin is a more stable form of (-)-Blebbistatin

Formal name: 1,2,3,3a-tetrahydro-3aS-hydroxy-6-methyl-1-phenyl-4H-Pyrrolo[2,3-b]quinolin-4-one

Synonyms:  (S)-Blebbistatin

Molecular weight: 292.3

CAS: 856925-71-8

Purity: ≥98%

Formulation: A crystalline solid

Product Type|Biochemicals|Small Molecule Inhibitors|ATPases||Product Type|Biochemicals|Transporter & Exchanger Modulators||Research Area|Cell Biology|Cell Death|Apoptosis||Research Area|Cell Biology|Cytoskeleton & Motor Proteins

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