A widely-used fluorescent indicator for estimating intracellular pH; amenable to either spectrofluorometry or flow cytometry; can also be used to investigate intracellular changes in other ions

BCECF AM – 5 mg

Brand:
Cayman
CAS:
117464-70-7
Storage:
-20
UN-No:
Non-Hazardous - /

BCECF AM ester is a widely-used fluorescent indicator for estimating intracellular pH.{25713,25712} This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours.{25710} Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm.{25712,25711} This approach is amenable to either spectrofluorometry or flow cytometry.{25712,25709} BCECF AM can also be used to investigate intracellular changes in other ions, including potassium.{25708}  

 

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Description

A widely-used fluorescent indicator for estimating intracellular pH; amenable to either spectrofluorometry or flow cytometry; can also be used to investigate intracellular changes in other ions, including potassium

Formal name: 3′,6′-bis(acetyloxy)-5(or 6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-spiro[isobenzofuran-1(3H),9′-[9H]xanthene]-2′,7′-dipropanoic acid, 2′,7′-bis[(acetyloxy)methyl] ester

Synonyms:  BCECF Acetoxymethyl ester

Molecular weight: 880.7

CAS: 117464-70-7

Purity: ≥95%

Formulation: An oil

Application|Flow Cytometry||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Dyes, Stains, & Indicators||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area

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