Immunogen: Mixture of a peptide with 6xHis-tag at the N-terminus and another peptide with 6xHis-tag at the C-terminus • Host: Rabbit • Species Reactivity: (+) All species • Cross Reactivity: (+) Recombinant proteins containing the 6xHis-tag

Anti-His-tag Rabbit Monoclonal Antibody (Clone RM146) – 100 µg

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Cayman
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Anti-His-tag Rabbit Monoclonal Antibody (Clone RM146) is a probe for immunochemical detection of His tags on recombinant proteins by western blot, immunoprecipitation, immunohistochemistry, immunocytochemistry, or flow cytometry. Recombinant proteins are routinely labeled with affinity tags, such as poly-histidine (poly-His), to facilitate both their detection and purification. Poly-His tags are often the tag of choice due to their small size, less potential to interfere in protein folding, weak immunogenicity, and high affinity for Ni2+.  

 

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SKU: 20725 - 100 µg Category:

Description

Immunogen: Mixture of a peptide with 6xHis-tag at the N-terminus and another peptide with 6xHis-tag at the C-terminus • Host: Rabbit • Species Reactivity: (+) All species • Cross Reactivity: (+) Recombinant proteins containing the 6xHis-tag, 10xHis-tag fused to either the amino or carboxy terminus; (-) Other endogenous protein in mammalian cells or bacteria • Application(s): WB, FC, ICC, IHC, and IP • A probe for immunochemical detection of His tags on recombinant proteins by FC, ICC, IHC, IP, or WB

Synonyms:

Immunogen: Mixture of a peptide with 6xHis-tag at the N-terminus and another peptide with 6xHis-tag at the C-terminus

Formulation: 100 µg of protein A affinity-purified antibody from an animal origin–free culture supernatant

Isotype: IgG

Applications: WB, FC, ICC, IHC, and IP

Origin:

Stability: 365 days

Application|Flow Cytometry||Application|Immunocytochemistry||Application|Immunohistochemistry||Application|Immunoprecipitation||Application|Western Blot||Product Type|Antibodies|Monoclonal Antibodies

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