A fluorescent cationic dye; ex/em spectra = 502/525

Acridine Orange – 5 g

Brand:
Cayman
CAS:
494-38-2
Storage:
-20
UN-No:
Non-Hazardous - /

Acridine orange is a cell-permeable, nucleic acid-selective fluorescent cationic dye useful for cell cycle determination and detection of cellular autophagy.{22781,22782,41255} It exhibits excitation/emission spectra of 502/525, 460/650, and 475/590 nm when bound to dsDNA, bound to ssDNA or RNA, and under acidic conditions, respectively.{22781,22782} The ratio of acridine orange emission at 590 to emission at 525 nm can be used to quantify the increase in the number of acidic vesicular organelles observed during cellular autophagy.  

 

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Description

A fluorescent cationic dye; ex/em spectra = 502/525, 460/650, and 475/590 nm when bound to dsDNA, bound to ssDNA or RNA, and under acidic conditions, respectively; ratio of emission at 590 to emission at 525 nm can be used to quantify the increase in acidic vesicular organelles observed during cellular autophagy

Formal name: N3,N3,N6,N6-tetramethyl-3,6-acridinediamine

Synonyms:  C.I. 46005B|NSC 194350

Molecular weight: 265.4

CAS: 494-38-2

Purity:

Formulation: A crystalline solid

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Cell Biology|Cell Cycle||Research Area|Cell Biology|Endomembrane System & Vesicular Trafficking|Autophagy

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