A para-nitro aniline chromophore cleaved by caspases (Km = 18

Ac-DEVD-pNA – 5 mg

Brand:
Cayman
CAS:
189684-50-2
Storage:
-20
UN-No:
Non-Hazardous - /

During apoptosis, activated caspase-3 cleaves several substrates, including poly(ADP-ribose) polymerase, which it specifically targets at the amino sequence DEVD.{22861} Ac-DEVD-pNA is a para-nitro aniline chromophore cleaved by caspases (Km = 18, 11, 32, 180, and 12 µM for caspases-1, -3, -4, -6, and -7, respectively).{7213} It is not cleaved by caspase-2.{7213} Cleavage is monitored colorimetrically at 405 nm.  

 

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Description

A para-nitro aniline chromophore cleaved by caspases (Km = 18, 11, 32, 180, and 12 µM for caspases-1, -3, -4, -6, and -7, respectively); not cleaved by caspase-2; monitored colorimetrically at 405 nm

Formal name: N-acetyl-L-α-aspartyl-L-α-glutamyl-L-valyl-N-(4-nitrophenyl)-L-α-asparagine

Synonyms:  Ac-Asp-Glu-Val-Asp-pNA

Molecular weight: 638.6

CAS: 189684-50-2

Purity: ≥95%

Formulation: A crystalline solid

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Dyes, Stains, & Indicators||Product Type|Biochemicals|Labeling & Detection|Enzyme Substrates||Research Area|Cell Biology|Cell Death|Apoptosis||Research Area|Cell Biology|Cell Death|Pyroptosis||Research Area|Cell Biology|Proteolysis|Cytosolic & Secreted Proteases

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