A fluorogenic substrate for activated caspase-3 (Km = 9.7 µM)

Ac-DEVD-AFC – 1 mg

Brand:
Cayman
CAS:
201608-14-2
Storage:
-20
UN-No:
Non-Hazardous - /

During apoptosis, activated caspase-3 cleaves several substrates, including poly(ADP-ribose) polymerase, which it specifically targets at the amino sequence DEVD.{22861} Ac-DEVD-AFC is a fluorogenic substrate for activated caspase-3 (Km = 9.7 µM), as well as related caspases.{22863,6382,22862} Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.  

 

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Description

A fluorogenic substrate for activated caspase-3 (Km = 9.7 µM), as well as related caspases; caspase activity can be quantified by fluorescent detection of free AFC, which is excited at 400 nm and emits at 505 nm

Formal name: N-acetyl-L-α-aspartyl-L-α-glutamyl-L-valyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-α-asparagine

Synonyms:  Caspase-3 Substrate (Fluorogenic)|N-Acetyl-Asp-Glu-Val-Asp-7-amino-4-Trifluoromethylcoumarin

Molecular weight: 729.6

CAS: 201608-14-2

Purity: ≥98%

Formulation: A crystalline solid

Application|Enzyme Activity||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Enzyme Substrates||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Product Type|Biochemicals|Small Molecule Inhibitors|Caspases||Product Type|Biochemicals|Small Molecule Inhibitors|MMPs||Research Area|Cell Biology|Cell Death|Apoptosis||Research Area|Cell Biology|Proteolysis|Cytosolic & Secreted Proteases

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