An internal standard for the quantification of 7-keto cholesterol by GC- or LC-MS

7-keto Cholesterol-d7 – 1 mg

Brand:
Cayman
CAS:
127684-08-6
Storage:
-20
UN-No:
Non-Hazardous - /

7-keto Cholesterol-d7 is intended for use as an internal standard for the quantification of 7-keto cholesterol (Item No. 16339) by GC- or LC-MS. 7-keto Cholesterol is a bioactive sterol and a major oxysterol component of oxidized LDL.{26766,5829} It is produced by oxidation of cholesterol via ethanol-mediated lipid peroxidation or photodamage as well as oxidation of 7-dehydro cholesterol (Item No. 14612) by the cytochrome P450 (CYP) isoform CYP7A1.{26764,26765,26761} 7-keto Cholesterol inhibits CYP7A1 (IC50 = ~1 μM).{26765} It induces activation and chemotaxis of retinal microglia as well as polarization to a pro-inflammatory state via NLRP3 inflammasome activation in vitro.{39975} Intraocular implantation of 7-keto cholesterol-coated wafers increases ocular levels of VEGF, IL-1β, and GRO/KC, macrophage infiltration, and neovascularization in rat eye.{39976} Levels of 7-keto cholesterol in lipid deposits are increased in a variety of chronic diseases, including atherosclerosis, Alzheimer’s disease, and age-related macular degeneration.  

 

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SKU: 25972 - 1 mg Category:

Description

An internal standard for the quantification of 7-keto cholesterol by GC- or LC-MS,

Formal name: 3β-hydroxy-cholest-5-en-7-one-25,26,26,26,27,27,27-d7

Synonyms:  7-oxo Cholesterol-d7|Δ5-Cholesterol-3β-ol-7-one-d7

Molecular weight: 407.7

CAS: 127684-08-6

Purity: ≥99% deuterated forms (d1-d7)

Formulation: A solid

Application|Mass Spectrometry||Product Type|Biochemicals|Analytical Standards||Product Type|Biochemicals|Isotopically Labeled Standards|Deuterium||Product Type|Biochemicals|Lipids|Sterol Lipids||Research Area|Cardiovascular System|Cardiovascular Diseases|Atherosclerosis||Research Area|Cardiovascular System|Lipids & Lipoproteins|Lipoproteins||Research Area|Immunology & Inflammation|Innate Immunity||Research Area|Lipid Biochemistry|Cytochrome P450 Pathways||Research Area|Lipid Biochemistry|Sterol Lipids||Research Area|Neuroscience|Ophthalmology||Research Area|Oxidative Stress & Reactive Species|Lipid Peroxidation

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