A nucleoside analog; inhibits replications of HSV-1 KOS strain (ID50 = 0.2 μg/mL); reduces virus-induced cytopathogenicity of HSV-1

5′-Ethynyl-2′-deoxycytidine – 5 mg

Brand:
Cayman
CAS:
69075-47-4
Storage:
-20
UN-No:
Non-Hazardous - /

5′-Ethynyl-2′-deoxycytidine (EdC) is a nucleoside analog that inhibits replication of the herpes simplex virus-1 (HSV-1) KOS strain (ID50 = 0.2 μg/mL).{36600} It also reduces virus-induced cytopathogenicity of HSV-1, HSV-2, and vaccinia virus strains in PRK cells (MICs = 0.2-0.4, 1-2, and 5 μg/ml, respectively).{36599} EdC is an inhibitor of thymidylate synthetase, selectively reducing DNA incorporation of [1′,2′-3H]deoxyuridine over [CH3-3H]deoxythymidine in PRK cells (ID50s = 3 and 120 μg/ml, respectively). It inhibits thymidine synthetase in and reduces proliferation of L1210 cells, an effect which is reversed by addition of deoxythymidine (ID50s = 4.4 and 1,000 μg/ml, respectively). EdC has been used to monitor DNA synthesis and cellular replication via click chemistry conjugation of the ethynyl group to an azido group of various fluorochromes.{36601}  

 

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Description

A nucleoside analog; inhibits replications of HSV-1 KOS strain (ID50 = 0.2 μg/mL); reduces virus-induced cytopathogenicity of HSV-1, HSV-2, and vaccinia virus strains in PRK cells (MICs = 0.2-0.4, 1-2, and 5 μg/ml, respectively); selectively inhibits thymidylate synthetase (ID50s = 3 and 120 μg/ml for incorporation of [1′,2′-3H]deoxyuridine and [CH3-3H]deoxythymidine, respectively); has been used to monitor DNA synthesis and cellular replication via click chemistry conjugation to fluorochromes,

Formal name: 2′-deoxy-5-ethynyl-cytidine

Synonyms:  2′-deoxy-5-Ethynylcytidine|EdC

Molecular weight: 251.2

CAS: 69075-47-4

Purity: ≥98%

Formulation: A crystalline solid

Product Type|Biochemicals|Antivirals||Product Type|Biochemicals|Nucleotides/Nucleosides||Product Type|Biochemicals|Small Molecule Inhibitors|Nucleic Acid Turnover/Signaling||Research Area|Epigenetics, Transcription, & Translation|RNA, DNA, & Protein Synthesis||Research Area|Infectious Disease|Viral Diseases|HSV

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