An amine-reactive fluorescent probe; has been used in double site-directed peptide modification to label proteinase substrates for use in FRET assays; quenches lucifer yellow fluorescence by 90% in uncleaved proteinase substrates

5-Carboxytetramethylrhodamine succinimidyl ester – 25 mg

Brand:
Cayman
CAS:
150810-68-7
Storage:
-20
UN-No:
- /

5-Carboxytetramethylrhodamine succinimidyl ester (5-TAMRA-SE) is an amine-reactive fluorescent probe.{53090} It has been used in double site-directed peptide modification to label proteinase substrates for use in FRET assays. It quenches lucifer yellow CH (Item No. 25573) fluorescence by greater than 90% in uncleaved proteinase substrates, which allows for the detection of enzyme-cleaved substrates by an increase in fluorescence intensity. It has also been used in the synthesis of fluorescent derivatives of a variety of compounds, including the antibiotic ampicillin (Item No. 14417), nucleotide diphosphate uridine-5’-diphosphate (UDP; Item No. 18137), and the progesterone receptor antagonist RU486 (mifepristone; Item No. 10006317).{53091,53092,53093} 5-TAMRA-SE displays excitation maxima ranging from 540 to 560 nm and an emission maximum of 580 nm.{53090}  

 

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SKU: 28509 - 25 mg Category:

Description

An amine-reactive fluorescent probe; has been used in double site-directed peptide modification to label proteinase substrates for use in FRET assays; quenches lucifer yellow fluorescence by 90% in uncleaved proteinase substrates, which allows for the detection of enzyme-cleaved substrates by an increase in fluorescence intensity; has also been used in the synthesis of fluorescent derivatives of a variety of compounds, including ampicillin, UDP, and RU486; ex/em = 540-560/580 nm, respectively

Formal name: 9-[2-carboxy-4-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-3,6-bis(dimethylamino)-xanthylium, inner salt

Synonyms:  5-TAMRA-SE

Molecular weight: 527.5

CAS: 150810-68-7

Purity: ≥95%

Formulation: A solid

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