A fluorogenic substrate of palmitoyl-protein thioesterase; cleaved by PPT/CLN1 to release the fluorescent moiety 4-MU; ex = 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH

4-Methylumbelliferyl 6-thio-Palmitate-β-D-Glucopyranoside – 5 mg

Brand:
Cayman
CAS:
229644-17-1
Storage:
-20
UN-No:
Non-Hazardous - /

4-Methylumbelliferyl 6-thio-palmitate-β-D-glucopyranoside is a fluorogenic substrate of palmitoyl-protein thioesterase (PPT, also known as CLN1), a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. 4-Methylumbelliferyl 6-thio-palmitate-β-D-glucopyranoside is cleaved by PPT/CLN1 to release the fluorescent moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases.{27251} This substrate is used in assays that measure PPT activity, which is commonly deficient in the neurodegenerative disorder known as infantile neuronal ceroid lipofuscinosis.{31634,31635}  

 

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Description

A fluorogenic substrate of palmitoyl-protein thioesterase; cleaved by PPT/CLN1 to release the fluorescent moiety 4-MU; ex = 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively; em = 445-455 nm, increasing as pH decreases,

Formal name: 4-methyl-7-[[6-S-(1-oxohexadecyl)-6-thio-β-D-glucopyranosyl]oxy]-2H-1-benzopyran-2-one

Synonyms:  Mu-6S-Palm-β-Glc

Molecular weight: 592.8

CAS: 229644-17-1

Purity: ≥95%

Formulation: A crystalline solid

Application|Enzyme Activity||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Enzyme Substrates||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Neuroscience|Neurodegenerative Disorders

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