A fluorogenic substrate for β-hexosaminidases; is cleaved by β-hexosaminidases to release the fluorescent moiety 4-ΜU; ex = 320 and 360 nm at low (1.97-6.72) and high pH (7.12-10.3)

4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-Glucopyranoside – 250 mg

Brand:
Cayman
CAS:
37067-30-4
Storage:
-20
UN-No:
Non-Hazardous - /

4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside is a fluorogenic substrate for β-hexosaminidases.{47303,47304} Upon enzymatic cleavage by β-hexosaminidases, 4-methylumbelliferone (4-ΜU) is released and its fluorescence can be used to quantify β-hexosaminidase activity. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high pH (7.12-10.3), respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases.{27251} 4-Methylumbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside has been used to quantify β-hexosaminidase activity in serum or leukocytes from patients with GM2 gangliosidoses such as Tay-Sachs disease, which is characterized by defects in the α subunit of β-hexosaminidase.{47304,40891}  

 

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SKU: 26953 - 250 mg Category:

Description

A fluorogenic substrate for β-hexosaminidases; is cleaved by β-hexosaminidases to release the fluorescent moiety 4-ΜU; ex = 320 and 360 nm at low (1.97-6.72) and high pH (7.12-10.3), respectively; em = 445 to 455 nm, increasing as pH decreases; has been used to quantify β-hexosaminidase activity in serum or leukocytes from patients with GM2 gangliosidoses such as Tay-Sachs disease

Formal name: 7-[[2-(acetylamino)-2-deoxy-β-D-glucopyranosyl]oxy]-4-methyl-2H-1-benzopyran-2-one

Synonyms:  4-Methylumbelliferyl-N-acetyl-β-Glucosaminide|4-ΜU-2-acetamido-2-deoxy-β-D-Glucopyranoside

Molecular weight: 379.4

CAS: 37067-30-4

Purity: ≥98%

Formulation: A crystalline solid

Application|Enzyme Activity||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Enzyme Substrates||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Neuroscience|Neurodegenerative Disorders

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