A fluorogenic substrate for α-L-iduronidase

4-Methylumbelliferyl-α-L-Iduronide (free acid) – 5 mg

Brand:
Cayman
CAS:
66966-09-4
Storage:
-20
UN-No:
Non-Hazardous - /

4-Methylumbelliferyl-α-L-iduronide (free acid) is a fluorogenic substrate for α-L-iduronidase, an enzyme found in cell lysosomes that is involved in the degradation of glycosaminoglycans such as dermatan sulfate and heparin sulfate. 4-Methylumbelliferyl-α-L-iduronide is cleaved by α-L-iduronidase to release the fluorescent moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases.{27251} This substrate is used in assays that measure the activity of α-L-iduronidase, which is commonly deficient in a type of lysosomal storage disease called mucopolysaccharidosis.{31700}  

 

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Description

A fluorogenic substrate for α-L-iduronidase, an enzyme found in cell lysosomes that is involved in the degradation of glycosaminoglycans; cleaved by α-L-iduronidase to release the fluorescent moiety 4-MU; ex = 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively; em = 445-455 nm, increasing as pH decreases

Formal name: 4-methyl-2-oxo-2H-1-benzopyran-7-yl α-L-idopyranosiduronic acid

Synonyms:  4-Methylumbelliferyl-α-L-Idopyranosiduronic Acid|4-MU-α-IdoA|MU-α-IdoA​

Molecular weight: 352.3

CAS: 66966-09-4

Purity: ≥90%

Formulation: A crystalline solid

Application|Enzyme Activity||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Enzyme Substrates||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Cell Biology|Endomembrane System & Vesicular Trafficking||Research Area|Endocrinology & Metabolism|Carbohydrate Metabolism

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