A fluorogenic substrate used to quantify GALNS activity; is cleaved by GALNS to release the fluorescent moiety 4-MU; ex = 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH

4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate (sodium salt) – 10 mg

Brand:
Cayman
CAS:
206443-06-3
Storage:
-20
UN-No:
Non-Hazardous - /

4-Methylumbelliferyl β-D-Galactopyranoside-6-sulfate (sodium salt) (4-MU-Gal-6S) is a fluorogenic substrate used to quantify N-acetylgalactosamine-6-sulphatase (GALNS) activity.{41061} 4-MU-Gal-6S is cleaved by GALNS to release the fluorescent moiety 4-MU. 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases.{27251} It has been used to detect Morquio disease type A, a lysosomal storage disorder in which GALNS is deficient.{41061} 4-MU-Gal-6S can be used to assess GALNS activity in a very small blood volume to determine the extent of deficiency.{41060}  

 

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Description

A fluorogenic substrate used to quantify GALNS activity; is cleaved by GALNS to release the fluorescent moiety 4-MU; ex = 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively; em = 445-455 nm, increasing as pH decreases; used to detect Morquio disease type A in an assay using a very small blood volume

Formal name: 4-methyl-7-[(6-O-sulfo-β-D-galactopyranosyl)oxy]-2H-1-benzopyran-2-one, monosodium salt

Synonyms:  4-Methylumbelliferyl Gal-6S|4-MU β-D-galactopyranoside-6-sulfate|4-MU-Gal-6S

Molecular weight: 440.4

CAS: 206443-06-3

Purity: ≥98%

Formulation: A crystalline solid

Application|Enzyme Activity||Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Enzyme Substrates||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Endocrinology & Metabolism|Carbohydrate Metabolism||Research Area|Endocrinology & Metabolism|Inborn Errors of Metabolism

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