A fluorescent probe for monitoring intracellular pH by flow cytometry in living cells; excited at UV wavelengths; ex/em spectra = 351/450-476 nm

3,6-diacetoxy Phthalonitrile – 25 mg

Brand:
Cayman
CAS:
83619-73-2
Storage:
-20
UN-No:
Non-Hazardous - /

3,6-diacetoxy Phthalonitrile is a fluorescent probe for monitoring intracellular pH by flow cytometry in living cells.{29427} It rapidly crosses cell membranes and is cleaved by cytosolic esterases to form the fluorescent pH indicator 2,3-dicyano-hydroquinone (DCH), which is excited at UV wavelengths. DCH can be excited at 351 nm, with pH-dependent emission in the range of 450-476 nm (pH 5.0-10.0) using a potassium-based buffer.{29427}  

 

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Description

A fluorescent probe for monitoring intracellular pH by flow cytometry in living cells; excited at UV wavelengths; ex/em spectra = 351/450-476 nm, with emission dependent upon pH

Formal name: 3,6-bis(acetyloxy)-1,2-benzenedicarbonitrile

Synonyms:  1,4-Diacetoxy-2,3-dicyanobenzene|2,3-Dicyano-1,4-hydroquinone diacetate|ADB

Molecular weight: 244.2

CAS: 83619-73-2

Purity: ≥98%

Formulation: A crystalline solid

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Dyes, Stains, & Indicators||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Research Area|Cell Biology

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