A fluorescently tagged cholesterol with the hydrophilic NBD fluorophore attached to carbon 3

3-hexanoyl-NBD Cholesterol – 500 µg

Brand:
Cayman
CAS:
201731-19-3
Storage:
-20
UN-No:
Non-Hazardous - /

3-hexanoyl-NBD cholesterol is a fluorescently tagged cholesterol derivative with the hydrophilic NBD fluorophore attached to carbon 3, at the hydrophilic end of cholesterol, separated by a 6-carbon spacer. This design allows the cholesterol to properly orient in membrane bilayers while the fluorescent tag is presented outside of the bilayer. This should model the behavior of cholesterol in membranes better than the previously-used 25-NBD cholesterol, which positions NBD directly on the 25th carbon of cholesterol at the hydrophobic terminus. 3-hexanoyl-NBD cholesterol has excitation/emission maxima of 473/536 nm, respectively, in vesicles comprised of dioleolylphosphatidylcholine (DOPC), however, the maxima will vary depending on membrane composition.{41361} NBD has excitation/emission maxima of 465/535 nm, respectively. Fluorescently tagged lipids have been used to study their interactions with proteins, their utilization by cells and liposomes, and for the development of assays for lipid metabolism.{16838,12304,16839,13590,14063}  

 

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Description

A fluorescently tagged cholesterol with the hydrophilic NBD fluorophore attached to carbon 3, at the hydrophilic end of cholesterol, separated by a 6-carbon spacer; allows the cholesterol to properly orient in membrane bi-layers while the fluorescent tag is presented outside of the bi-layer; ex/em = 473/536 nm in vesicles comprised of DOPC but will vary depending on membrane composition

Formal name: 6-[(7-nitro-2,1,3-benzoxadial-4-yl)amino]-cholest-5-en-3-ol

Synonyms:  3-C6-NBD Cholesterol

Molecular weight: 662.9

CAS: 201731-19-3

Purity: ≥98%

Formulation: A crystalline solid

Application|Fluorescence||Product Type|Biochemicals|Labeling & Detection|Fluorescent Probes||Product Type|Biochemicals|Lipids|Sterol Lipids||Research Area|Lipid Biochemistry|Sterol Lipids

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